Assessing dentinal tubule penetration of an innovative bioactive glass-based root canal sealer through confocal laser scanning microscopy: an in vitro analysis

Submitted: 10 January 2024
Accepted: 28 February 2024
Published: 26 March 2024
Abstract Views: 702
PDF: 74
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Aim: To assess and compare the dentinal tubule penetration of zinc oxide eugenol (ZOE)-based, resin-based, bioceramic, and novel bioactive glass-based root canal sealers using a confocal laser scanning microscope (CLSM).

Methods: A total of 48 single-rooted permanent teeth were categorized into four groups (n=12) and treated with gutta-percha along with ZOE sealer (Tubli-Seal EWT), resin-based sealer (AH Plus), bioceramic sealer (BioRoot RCS), and bioactive glass (Nishika Canal Sealer-BG). Cross-sections of the roots at 3 mm and 6 mm from the apex were examined under a CLSM to evaluate dentinal tubule penetration.

Results: Results indicated that bioceramic sealers exhibited the highest depth of dentinal tubule penetration at both levels, followed by bioactive glass and resin-based sealers. ZOE-based sealer demonstrated the least tubule penetration. Bioactive glass displayed the highest percentage of sealer penetration at 3 mm and 6 mm, with no statistically significant difference observed between bioceramic and bioactive glass groups regarding depth and percentage of dentinal tubule penetration at both levels.

Conclusions: Of particular significance is the bioactive glass group, demonstrating the most substantial sealer penetration percentage compared to the other groups at both examined depths.

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Barbhai, S., SR, S., Shetty, R., Joshi, P., Mehta, V., & Meto, A. (2024). Assessing dentinal tubule penetration of an innovative bioactive glass-based root canal sealer through confocal laser scanning microscopy: an in vitro analysis. Giornale Italiano Di Endodonzia, 38(2). https://doi.org/10.32067/GIE.2024.38.01.06