https://doi.org/10.32067/GIE.2021.35.02.52

Microbiological analysis of root canals of primary teeth with pulp necrosis caused by caries or trauma

Vol. 36 No. 1 (2022)
Submitted: 10 November 2021
Accepted: 24 February 2022
Published: 2 May 2022
Trauma; Dental caries; Microorganisms; Polymerase chain reaction; Endodontics.
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Authors

Denise L. Sousa
School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
Rebecca B.R. Araújo
School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
Brenda P.F.A. Gomes
bpgomes@fop.unicamp.br
Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
Juliana D. Bronzato
Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
Thais M. Duque
Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP and School of Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Juliana O. Gondim
School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
José J. S. Moreira-Neto
School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.

Aim: This study aimed to investigate the microorganisms detected from root canals of primary teeth with pulp necrosis caused by dental caries or trauma.

Methodology: Microbial samples were taken from 44 cases in primary teeth with pulp necrosis either due to dental caries or trauma. DNA was extracted from the samples, which were analysed for the presence of fifteen endodontic pathogens by using PCR species-specific primers.

Results: The bacteria most detected in necrotic primary teeth due to caries (37/44) were P. micra (76.3%), P. nigrescens (76.3%), A. naeslundii (47.4%), and F. nucleatum (42.1%). On the other hand, A. naeslundii (83.3%), T. forsythia (83.3%), P. nigrescens (66.7%), and F. nucleatum (66.7%) were most frequently recovered from a root canal with pulp necrosis due to trauma (7/44). Significant associations were found between the presence of P. micra and the existence of caries (p=0.023) and sinus tract (p=0.044). The presence of T. forsythia was associated with the existence of trauma (p=0.035), and the presence of F. nucleatum was associated with pain on palpation (p=0.033).

Conclusions: The microbiota recovered from root canals of primary teeth with pulp necrosis caused by dental caries or trauma is similar, with the predominance of anaerobic microorganisms.

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Supporting Agencies

Brazilian Agencies CAPES [financial code 001], CNPQ [308162/2014-5] and FAPESP [2015/23479-5].

How to Cite

L. Sousa, D., B.R. Araújo, R., Gomes, B. P., D. Bronzato, J., M. Duque, T., O. Gondim, J., & J. S. Moreira-Neto, J. (2022). Microbiological analysis of root canals of primary teeth with pulp necrosis caused by caries or trauma. Giornale Italiano Di Endodonzia, 36(1). https://doi.org/10.32067/GIE.2021.35.02.52
Copyright (c) 2022 Denise L. Sousa, Rebecca B.R. Araújo, Brenda P.F.A. Gomes, Juliana D. Bronzato, Thais M. Duque, Juliana O. Gondim, José J. S. Moreira-Neto Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Giornale Italiano di Endodonzia has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.