Immunohistochemical analysis of Biodentine versus MTA in repair of furcation perforation: an animal study

Aim: This study compared Biodentine (BD) and mineral trioxide aggregate (MTA Angelus) in repair of furcation perforation (FP) in terms of inflammation and new hard tissue formation.

Methodology: Ninety six teeth in six adult mongrel dogs were divided into two equal groups (48 teeth/3 dogs each) according to the time of repair; immediate and delayed repair of the induced FP. These groups were divided into three subgroups (16 teeth each) according to the evaluation period; 1, 2, and 3 months. Each subgroup was further subdivided into four subdivisions according to the FP repair material used; MTA Angelus (6 teeth), BD (6 teeth), negative control (2 teeth) and positive control (2 teeth). Instrumentation, obturation and FP were performed in the experimental and positive control teeth. The perforations were sealed according to the groups and subdivisions. Histopathology and immunohistochemical analysis using Osteonectin antibodies were performed for assessment of the inflammatory cell count and new hard tissue formation. All data were statistically analyzed.

Results: In all groups and subgroups, there were no significant differences between MTA and BD in the inflammatory cell count and new hard tissue formation (P>0.05).

Conclusion: Compared to MTA Angelus as a FP repair material, Biodentine induces similar degree of inflammation and new hard tissue formation.